德晋贵宾厅

• TL1A is a TNF superfamily ligand that binds to death receptor 3 (DR3) and delivers potent costimulatory signals, regulating effector cell proliferation, activation, apoptosis, and the production of cytokines and chemokines. Soluble decoy receptor 3 (DcR3) can neutralize sTL1A/DR3 signaling and inhibit apoptosis, dampen inflammation, and limit tissue damage by neutralizing LIGHT and FasL.
• IL-23 is a heterodimeric cytokine composed of p40 and p19 subunits, mainly secreted by macrophages and dendritic cells. By binding to its receptor (IL-23R), IL-23 drives downstream proinflammatory cytokine release and plays a central role in Th17-mediated pathology observed in multiple autoimmune diseases.
• In TL1A/IL23A/IL12B humanized mice, the extracellular domain–encoding region of the mouse Tl1a gene is replaced by its human TL1A counterpart, and the full-length mouse Il23a and Il12b coding regions are replaced with human IL23A and IL12B, respectively. Soluble human TL1A and human IL-23 are exclusively detectable in homozygous TL1A/IL23A/IL12B humanized mice but not in wild-type (WT) mice. Humanization of TL1A, IL23A, and IL12B does not alter immune-cell frequency or distribution in the spleen, blood, or lymph nodes, nor does it affect blood-cell composition, morphology, liver enzymes (ALT, AST), or overall liver health.

Key Advantages

• Triple humanized cytokine design enabling simultaneous evaluation of TL1A-, IL-23-, and IL-12–targeting therapies.
• Physiological expression under endogenous mouse promoters ensures natural regulation and biological relevance.
• Normal immune cell homeostasis in spleen, blood, and lymph nodes.
• Active human cytokine signaling, producing soluble human TL1A and IL-23 from dendritic cells.
• Robust colitis phenotype induced by TNBS, including inflammation, cytokine elevation, and fibrosis.
• Ideal for IBD preclinical studies, colitis drug evaluation, mechanistic cytokine pathway research, and combination antibody therapy testing.

Application

• In vivo evaluation of anti-human TL1A, anti-human IL23p19, and combination biologics
• Mechanistic studies of the TL1A–IL23–IL12 cytokine axis
• TNBS-induced acute colitis and fibrosis
• IBD drug discovery and efficacy assessment
• Cytokine stimulation studies in dendritic cells
• Modeling TL1A/IL23/IL12–driven inflammation

Species-specific analysis of TL1A, IL23A and IL12B gene expression in wild-type C57BL/6 mice and homozygous TL1A/IL23A/IL12B humanized mice was performed by RT-PCR. Colon and lung tissues were collected from wild-type mice C57BL/6 (+/+) and homozygous humanized mice (H/H). Mouse Tl1a, Il23a and Il12b mRNA were detectable only in wild-type mice, whereas human TL1A, IL-23A and IL-12B transcripts were detectable only in homozygous TL1A/IL23A/IL12B humanized mice.

Soluble TL1A expression analysis in TL1A/IL23A/IL12B humanized mice was performed by ELISA. Bone marrow-derived dendritic cells from wild-type and humanized mice (female, 9-week-old, n = 3) were stimulated with LPS. Supernatants were collected and analyzed for soluble TL1A. Human TL1A was exclusively detectable in TL1A/IL23A/IL12B humanized mice; mouse TL1A was detectable only in wild-type mice. Values are expressed as mean ± SEM. ND = not detectable.

Strain-specific IL-23 expression analysis in TL1A/IL23A/IL12B humanized mice. Bone marrow-derived dendritic cells were produced by culturing the bone marrow from wild-type C57BL/6 mice and homozygous B-hTL1A/hIL23A/hIL12B mice (female, 6-week-old, n=3), which were stimulated with LPS in vitro. After stimulation, the supernatants were collected, and the levels of mouse and human IL-23 were analyzed by ELISA. Mouse IL-23 was only detectable in wild-type C57BL/6 mice, whereas human IL-23 was exclusively detectable in homozygous TL1A/IL23A/IL12B humanized mice. Values are expressed as mean ± SEM. ND = not detectable.

Frequency of leukocyte subpopulations in spleen was analyzed by flow cytometry. Splenocytes were isolated from wild-type and TL1A/IL23A/IL12B humanized mice (female, 7-week-old, n = 3). Flow cytometry analysis was performed to quantify leukocyte subsets and T-cell subpopulations. Percentages of T cells, B cells, NK cells, DCs, monocytes, macrophages, neutrophils, CD4⁺ T cells, CD8⁺ T cells and Tregs in humanized mice were comparable to wild-type mice. Values are expressed as mean ± SEM.

Blood leukocyte subpopulation profiling was performed via flow cytometry using samples from wild-type and TL1A/IL23A/IL12B humanized mice (female, 7-week-old, n = 3). Leukocyte and T-cell subset frequencies (CD4⁺, CD8⁺ and Tregs) were similar between groups, indicating that humanization of TL1A, IL23A and IL12B does not change the frequency or distribution of these cell types in the blood. Values are expressed as mean ± SEM.

Lymph-node leukocyte profiling was performed using samples from wild-type and TL1A/IL23A/IL12B humanized mice (female, 7-week-old, n = 3). Frequencies of T cells, B cells, NK cells, CD4⁺ T cells, CD8⁺ T cells and Tregs were comparable between genotypes, indicating that humanization of TL1A, IL23A and IL12B does not change the frequency or distribution of these cell types in lymph nodes. Values are expressed as mean ± SEM.

Growth curve of wild-type C57BL/6 and B-hTL1A/hIL23A/hIL12B mice. 8-week-old mice were grouped (10 males and 10 females, respectively). Body weight was measured on the same day of every two week, until 32 weeks. The lowest and highest values of mouse body weight in the table were calculated from the mean ± SD. The growth curve of the B-hTL1A/hIL23A/hIL12B mice was similar to the growth curve of C57BL/6 mice. 

Complete blood count (CBC) of B-hTL1A/hIL23A/hIL12B mice. Values are expressed as mean ± SD.

Complete blood count (CBC) of B-hTL1A/hIL23A/hIL12B mice. Values are expressed as mean ± SD.

Complete blood count (CBC) of B-hTL1A/hIL23A/hIL12B mice. Values are expressed as mean ± SD.

Complete blood count (CBC) of B-hTL1A/hIL23A/hIL12B mice. Values are expressed as mean ± SD.

Biochemical test of B-hTL1A/hIL23A/hIL12B mice. Values are expressed as mean ± SD.

Biochemical test of B-hTL1A/hIL23A/hIL12B mice. Values are expressed as mean ± SD.

Biochemical test of B-hTL1A/hIL23A/hIL12B mice. Values are expressed as mean ± SD.

Biochemical test of B-hTL1A/hIL23A/hIL12B mice. Values are expressed as mean ± SD.

Histopathological analysis of organs in female C57BL/6 and B-hTL1A/hIL23A/hIL12B mice. The main organs of C57BL/6 and B-hTL1A/hIL23A/hIL12B mice were isolated at 32 weeks of age and analyzed with H&E staining (female, n=3). Results showed that no obvious abnormalities were found in all of the organs (brain, heart, lung, liver, spleen, stomach, small intestine, large intestine, kidney, uterus and ovary).

Histopathological analysis of organs in male C57BL/6 and B-hTL1A/hIL23A/hIL12B mice. The main organs of C57BL/6 and B-hTL1A/hIL23A/hIL12B mice were isolated at 32 weeks of age and analyzed with H&E staining (male, n=3). Results showed that no obvious abnormalities were found in all of the organs (brain, heart, lung, liver, spleen, stomach, small intestine, large intestine, kidney, and testis).

TNBS solution was instilled into the colonic lumen of TL1A/IL23A/IL12B humanized mice (female, 8–10 weeks old, n = 10). Sham controls received 50% ethanol. Treatment groups received anti-human TL1A antibody Tulisokibart (10 mpk, WuXi AppTec) and anti-human IL-23p19 antibody Risankizumab (10 mpk, WuXi AppTec), administered alone or in combination. Body weight and DAI were recorded daily. On day 5, mice were sacrificed and colon length and weight were measured.(A) Body-weight change. (B) DAI score. (C) Colon index. (D) Colon images. The TNBS model was successfully established. Both antibodies significantly alleviated acute colitis, and combination therapy showed superior efficacy. TL1A/IL23A/IL12B humanized mice constitute a robust model for evaluating anti-human TL1A and anti-human IL-23p19 antibodies in vivo. Values are expressed as mean ± SEM.*p < 0.05, **p < 0.01, ***p < 0.001, ***p < 0.0001 vs vehicle, ANOVA.

TNBS-induced colonic pathology and fibrosis in TL1A/IL23A/IL12B humanized mice. Using the same dosing regimen, colon tissues were collected on day 5 for H&E and Masson staining. (A) Pathological score. (B) Masson fibrosis score. Both antibodies significantly reduced epithelial damage and fibrotic progression, with combination therapy providing enhanced protection. Values are expressed as mean ± SEM. *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001 vs Vehicle, ANOVA.

Note: Study conducted by WuXi AppTec.

Cytokine analysis in TNBS-induced acute colitis in TL1A/IL23A/IL12B humanized mice. Following the same treatment protocol, mucosa and serum were collected on day 5 for cytokine assessment. (A) IL-1β, IL-6, IL-17A, IFN-γ, TNF-α in intestinal mucosa. (B) IL-1β, IL-6, IL-17A, IFN-γ, TNF-α in serum. Anti-human TL1A and anti-human IL-23p19 antibodies significantly reduced inflammatory cytokine levels, and combination therapy provided the strongest inhibition, highlighting the model’s utility for preclinical testing of human-specific TL1A/IL23 pathway therapeutics. Values are expressed as mean ± SEM. *p<0.05, **p<0.01, ***p<0.001, ***p<0.0001 vs Vehicle, ANOVA.

Note: Study conducted by WuXi AppTec.

Q1. What are TL1A/IL23A/IL12B humanized mice?

A triple-cytokine humanized strain expressing human TL1A, IL-23A and IL-12B for IBD and colitis research.

Q2. What studies are these mice best suited for?

TNBS-induced colitis, cytokine biology, fibrosis, immune pathway analysis, and evaluation of anti-human TL1A, IL-23, and IL-12B antibodies.

Q3. Do these mice maintain normal immune homeostasis?

Yes. Leukocyte and T-cell distributions in spleen, blood and lymph nodes remain comparable to wild-type mice.

Q4. Can human-specific antibodies be directly tested?

Yes. Anti-human TL1A and anti-human IL-23p19 antibodies were tested and showed robust in vivo efficacy in this model.

Q5. Are these mice suitable for combination therapy studies?

Yes. The model supports evaluation of combination therapy targeting TL1A and IL-23 pathways.