• 德晋贵宾厅

    B-hSIGLEC6 mice(C)

    BALB/cCrSlcNifdc-Igs2tm1(SIGLEC6)Bcgen/Bcgen • 113333

    B-hSIGLEC6 mice(C)

    Product nameB-hSIGLEC6 mice(C)
    Catalog number113333
    Strain nameBALB/cCrSlcNifdc-Igs2tm1(SIGLEC6)Bcgen/Bcgen
    Strain backgroundBALB/cCrSlcNifdc
    NCBI gene ID946 (Human)
    AliasesCD327; CD33L; OBBP1; CD33L1; CD33L2; CDW327

    在此页面上

    • Description
    • Targeting strategy
    • Phenotypic analysis

    海报

    查看全部

      发表文章

        Description
        • SIGLEC6 is an inhibitory receptor selectively expressed on human mast cells and is a member of a family of receptors that bind to sialoglycan.
        • The full coding CDS, the promoter, 5’UTR and 3’UTR sequences of human SIGLEC6 gene are inserted into mouse Hipp11 (H11) locus in B-hSIGLEC6 mice(C).
        • Human SIGLEC6 mRNA  was exclusively detectable in homozygous B-hSIGLEC6 mice(C).
        • Human SIGLEC6 protein was detectable on mast cells in the peritoneal lavage fluid, B cells, NK cells, DCs, neutrophils, monocytes and macrophages in the blood of homozygous B-hSIGLEC6 mice(C).
        • Application: This product is used for pharmacodynamics and safety evaluation of  drugs.
        Targeting strategy

        Gene targeting strategy for B-hSIGLEC6 mice(C). The full coding CDS, the promoter, 5’UTR and 3’UTR sequences of human SIGLEC6 gene are inserted into mouse Hipp11 (H11) locus in B-hSIGLEC6 mice(C).

        mRNA expression analysis

        Species specific analysis of SIGLEC6 gene expression in wild-type BALB/c mice and homozygous humanized B-hSIGLEC6 mice(C) by RT-PCR. Spleen was collected from wild-type BALB/c mice (+/+) and homozygous B-hSIGLEC6 mice(C) (H/H). Human SIGLEC6 mRNA was detectable only in homozygous B-hSIGLEC6 mice(C), but not in wild-type BALB/c mice.

        Protein expression analysis in mast cells

        Strain specific SIGLEC6 expression analysis in homozygous B-hSIGLEC6 mice(C) by flow cytometry. Peritoneal lavage fluid was collected from wild-type BALB/c mice (+/+) and homozygous B-hSIGLEC6 mice(C) (H/H), and protein expression was analyzed with anti-human SIGLEC6 antibody (BD, 747915) by flow cytometry. hSIGLEC6 was only detectable in mast cells of homozygous B-hSIGLEC6 mice but not in wild-type BALB/c mice.

        Protein expression analysis in T, B and NK cells

        Strain specific SIGLEC6 expression analysis in homozygous B-hSIGLEC6 mice(C) by flow cytometry. Splenocytes, blood, and lymph nodes were collected from wild-type BALB/c mice (+/+) and homozygous B-hSIGLEC6 mice(C) (H/H), and protein expression was analyzed with anti-human SIGLEC6 antibody (BD, 747915) by flow cytometry. hSIGLEC6 was detectable on B and NK cells in the blood of homozygous B-hSIGLEC6 mice(C).

        Protein expression analysis in neutrophils and DCs

        Strain specific SIGLEC6 expression analysis in homozygous B-hSIGLEC6 mice(C) by flow cytometry. Splenocytes and blood were collected from wild-type BALB/c mice (+/+) and homozygous B-hSIGLEC6 mice(C) (H/H), and protein expression was analyzed with anti-human SIGLEC6 antibody (BD, 747915) by flow cytometry. hSIGLEC6 was only detectable on neutrophils and DCs in the blood of homozygous B-hSIGLEC6 mice( C).

        Protein expression analysis in monocytes and macrophages

        Strain specific SIGLEC6 expression analysis in homozygous B-hSIGLEC6 mice(C) by flow cytometry. Splenocytes and blood were collected from wild-type BALB/c mice (+/+) and homozygous B-hSIGLEC6 mice(C) (H/H), and protein expression was analyzed with anti-human SIGLEC6 antibody (BD, 747915) by flow cytometry. hSIGLEC6 was only detectable on monocytes and macrophages in blood of homozygous B-hSIGLEC6 mice(C).

        * When publishing results obtained using this animal model, please acknowledge the source as follows: The animal model [B-hSIGLEC6 mice(C)] (Cat# 113333) was purchased from Biocytogen.