C57BL/6-Igfbp2tm1(IGFBP2)Bcgen/Bcgen • 110987
| Product name | B-hIGFBP2 mice |
|---|---|
| Catalog number | 110987 |
| Strain name | C57BL/6-Igfbp2tm1(IGFBP2)Bcgen/Bcgen |
| Strain background | C57BL/6 |
| NCBI gene ID | 3485 (Human) |
| Aliases | IBP2; IGF-BP53 |
Gene targeting strategy for B-hIGFBP2 mice. The exons 1-4 of mouse Igfbp2 gene that encode the full-length protein were replaced by human IGFBP2 exons 1-4 in B-hIGFBP2 mice.
Strain specific IGFBP2 expression analysis in heterozygous B-hIGFBP2 mice by Western Blot. Liver tissue was collected from wild-type C57BL/6 mice (+/+) and heterozygous B-hIGFBP2 mice (H/+) , and analyzed by western blot with species-specific IGFBP2 antibody. Mouse IGFBP2 was detectable in wild type mice and heterozygous B-hIGFBP2 mice, as the antibody is crossly reactive with IGFBP2 in human and mice. Human IGFBP2 was exclusively detectable in heterozygous B-hIGFBP2 mice but not wild-type mice.
