C57BL/6-Icostm7(ICOS)Icosltm1(ICOSLG)/Bcgen • 112901
| Product name | B-hICOS/hICOSL mice |
|---|---|
| Catalog number | 112901 |
| Strain name | C57BL/6-Icostm7(ICOS)Icosltm1(ICOSLG)/Bcgen |
| Strain background | C57BL/6 |
| NCBI gene ID | 23308,29851 (Human) |
| Aliases | B7h; B7H2; GL50; B7-H2; B7RP1; CD275; ICOSL; LICOS; B7RP-1; ICOS-L; IMD119; AILIM; CD278; CVID1 |
Gene targeting strategy for B-hICOS/hICOSL mice. The exons 1-5 of mouse Icos gene that encode the full-length protein were replaced by human ICOS exons 1-5 in B-hICOS/hICOSL mice. The exons 3-5 of mouse IcosL gene that encode the extracellular domain were replaced by human ICOSLG exons 3-5 in B-hICOS/hICOSL mice.
Strain specific ICOS expression analysis in homozygous B-hICOS/hICOSL miceby flow cytometry. Splenocytes were collected from wild type (WT) mice (+/+) and homozygous B-hICOS/hICOSL mice (H/H; H/H) stimulated with anti-CD3ε in vivo and analyzed by flow cytometry with vopratelimab analog (in house), STIM-003 analog (in house) and GSK-3359609 analog (in house). ICOS was detectable in WT mice (+/+) and homozygous B-hICOS/hICOSL mice (H/H; H/H) due to the cross-reactivity of antibodies. Human ICOS was exclusively detectable in homozygous B-hICOS/hICOSL mice (H/H; H/H) but not in WT mice (+/+).
Strain specific ICOSL expression analysis in homozygous B-hICOS/hICOSL mice by flow cytometry. Splenocytes were collected from wild type (WT) mice (+/+) and homozygous B-hICOS/hICOSL mice (H/H; H/H) , and analyzed by flow cytometry with anti-human ICOSL antibody prezalumab analog (in house), antibody A and antibody B provided by the client. Human ICOSL was exclusively detectable in homozygous B-hICOS/hICOSL mice (H/H; H/H) but not in WT mice (+/+).
