C57BL/6-Hamptm1(HAMP)Bcgen/Bcgen • 111019
| Product name | B-hHAMP mice |
|---|---|
| Catalog number | 111019 |
| Strain name | C57BL/6-Hamptm1(HAMP)Bcgen/Bcgen |
| Strain background | C57BL/6N |
| NCBI gene ID | 57817 (Human) |
| Aliases | HEPC; PLTR; HFE2B; LEAP1 |
Gene targeting strategy for B-hHAMP mice. The exons 1~3 of mouse Hamp gene that encodes the full-length protein was replaced by human HAMP exons 1~3 in B-hHAMP mice.
Strain specific HAMP expression analysis in homozygous B-hHAMP mice by ELISA. Serum were isolated from wild-type C57BL/6N mice (+/+) and homozygous B-hHAMP mice (H/H), and analyzed by ELISA with species-specific HAMP ELISA kit. Human HAMP was exclusively detectable in homozygous B-hHAMP mice but not in wild-type mice.
Strain specific analysis of HAMP mRNA expression in wild-type C57BL/6 mice and B-hHAMP mice by RT-PCR. Liver RNA were isolated from wild-type C57BL/6N mice (+/+) and homozygous B-hHAMP mice (H/H), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mouse or human HAMP primers. Mouse Hamp mRNA were detectable in wild-type C57BL/6. Human HAMP mRNA was detectable in homozygous B-hHAMP mice.
