Targeting strategy
Gene targeting strategy for B-hFGL1 mice. The exons 2~8 of mouse Fgl1 gene that encode the full-length protein were replaced by human FGL1 exons 2~8 in B-hFGL1 mice.
mRNA expression analysis
Strain specific analysis of FGL1 gene expression in WT and heterozygous B-hFGL1 mice by RT-PCR. Mouse Fgl1 mRNA was detectable in liver cells of wild-type (+/+) mice and heterozygous B-hFGL1 mice. Human FGL1 mRNA was detectable only in H/+, but not in +/+ mice.
Protein expression analysis
Strain specific analysis of FGL1 expression in WT and B-hFGL1 mice by western blot. Liver were collected from WT mice and heterozygous B-hFGL1 (H/+) mice. Mouse FGL1 was detectable in WT mice and heterozygous B-hFGL1. Human FGL1 was exclusively detectable in heterozygous B-hFGL1 but not WT mice.
* When publishing results obtained using this animal model, please acknowledge the source as follows: The animal model [B-hFGL1 mice] (Cat# 110116) was purchased from Biocytogen.
