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    B-hEPHB3 mice

    C57BL/6N-Ephb3tm1(EPHB3)Bcgen/Bcgen • 111307

    B-hEPHB3 mice

    Product nameB-hEPHB3 mice
    Catalog number111307
    Strain nameC57BL/6N-Ephb3tm1(EPHB3)Bcgen/Bcgen
    Strain backgroundC57BL/6N
    NCBI gene ID2049 (Human)
    AliasesEK2; ETK2; HEK2; TYRO6

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    • Targeting strategy
    • Phenotypic analysis

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      发表文章

        Targeting strategy

        Gene targeting strategy for B-hEPHB3 mice. The exons 3~7 of mouse Ephb3 gene that encode the extracellular domain were replaced by human EPHB3 exons 3~7 in B-hEPHB3 mice.

        Protein expression analysis

        Western blot analysis of EPHB3 protein expression in homozygous B-hEPHB3 mice. Various tissue lysates were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hEPHB3 mice (H/H), and then analyzed by proteinsimple-western blot with anti-EPHB3 antibody. 3μg total proteins were loaded for western blotting analysis. The EPHB3 protein was detected in the lung, liver, stomach, brain of wild-type C57BL/6 mice and homozygous B-hEPHB3 mice, as confirmed by cross-reactivity of the antibody against mouse and human EPHB3 proteins.

        mRNA expression analysis
        Strain specific analysis of EPHB3 mRNA expression in wild-type C57BL/6 mice and B-hEPHB3 mice by RT-PCR. Stomach RNA were isolated from wild-type C57BL/6 mice (+/+) and homozygous B-hEPHB3 mice (H/H), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mouse or human EPHB3 primers. Mouse Ephb3 mRNA was detectable only in wild-type C57BL/6 mice. Human EPHB3 mRNA was detectable only in homozygous B-hEPHB3 mice but not in wild-type mice.
        * When publishing results obtained using this animal model, please acknowledge the source as follows: The animal model [B-hEPHB3 mice] (Cat# 111307) was purchased from Biocytogen.