德晋贵宾厅

    B-hADAM9 mice

    C57BL/6-Adam9tm1(ADAM9)Bcgen/Bcgen • 111342

    B-hADAM9 mice

    Product nameB-hADAM9 mice
    Catalog number111342
    Strain nameC57BL/6-Adam9tm1(ADAM9)Bcgen/Bcgen
    Strain backgroundC57BL/6
    NCBI gene ID8754 (Human)
    AliasesMCMP; MDC9; CORD9; Mltng

    在此页面上

    • Phenotypic analysis

    海报

    查看全部

      发表文章

        Protein expression analysis

        Western blot analysis of ADAM9 protein expression in homozygous B-hADAM9 mice. Various tissue lysates were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hADAM9 mice (H/H), and then analyzed by western blot with anti-ADAM9 antibody. 40 μg total proteins were loaded for western blotting analysis. ADAM9 protein was detected in the heart, liver, spleen, lung, kidney, brain and thymus of wild-type C57BL/6 mice and homozygous B-hADAM9 mice, as the antibody is cross-recognize both human and mouse ADAM9.

        mRNA expression analysis
        Strain specific analysis of ADAM9 mRNA expression in wild-type C57BL/6 mice and B-hADAM9 mice by RT-PCR. Brain and lung RNA were isolated from wild-type C57BL/6 mice (+/+) and homozygous B-hADAM9 mice (H/H), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mouse or human ADAM9 primers. Mouse Adam9 mRNA was detectable only in wild-type C57BL/6 mice. Human ADAM9 mRNA was detectable only in homozygous B-hADAM9 mice but not in wild-type mice. And the PCR products were confirmed with sanger sequencing.
        * When publishing results obtained using this animal model, please acknowledge the source as follows: The animal model [B-hADAM9 mice] (Cat# 111342) was purchased from Biocytogen.