• 321898
| Product name | B-Tg(mAreg) MC38 |
|---|---|
| Catalog number | 321898 |
| Strain background | C57BL/6 |
| NCBI gene ID | 11839 (Mouse) |
| Aliases | AR; Mcub; Sdgf |
| Tissue | Colon |
| Disease | Colon carcinoma |
| Species | Mouse |
| Application | B-Tg(mAreg) MC38 |
The exogenous promoter and mouse Areg coding sequence were inserted into the mouse genome randomly. Mouse Areg can be secreted by B-Tg(mAreg) MC38 cells.
Gene targeting strategy for B-Tg(mAreg) MC38 cells. The exogenous promoter and mouse Areg coding sequence were inserted into the mouse genome randomly.
Areg expression analysis in B-Tg(mAreg) MC38 cells by ELISA. Mouse Areg was detected in the supernatant of B-Tg(mAreg) MC38 cells. The 1-D01 clone of B-Tg(mAreg) MC38 cells was used for in vivo experiments.
Subcutaneous homograft tumor growth of B-Tg(mAreg) MC38 cells. B-Tg(mAreg) MC38 cells (5x105) and wild-type MC38 cells (5x105) were subcutaneously implanted into C57BL/6 mice (female, 6-week-old, n=6). Tumor volume and body weight were measured twice a week. (A) Average tumor volume ± SEM. (B) Body weight (Mean ± SEM). Volume was expressed in mm3 using the formula: V=0.5 X long diameter X short diameter2. As shown in panel A, B-Tg(mAreg) MC38 cells were able to establish tumors in vivo and can be used for efficacy studies.
B-Tg(mAreg) MC38 tumor growth of individual mice. B-Tg(mAreg) MC38 cells (5x105) and wild-type MC38 cells (5x105) were subcutaneously implanted into C57BL/6 mice (female, 6-week-old, n=6). As shown in panel, B-Tg(mAreg) MC38 cells were able to establish tumors in vivo and can be used for efficacy studies.
